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082 0 4 _a572.8
_bS187m 2001
100 1 _9273585
_aSambrook, Joseph,
_eautor
245 1 0 _aMolecular cloning :
_ba laboratory manual /
_cJoseph Sambrook, David W. Russell.
250 _a3rd ed.
264 3 1 _aCold Spring Harbor, N.Y. :
_bCold Spring Harbor Laboratory Press,
_cc2001.
300 _a3 volúmenes :
_bilustraciones
336 _atexto
_btxt
_2rdacontent
337 _ano mediado
_bn
_2rdamedia
338 _avolumen
_bnc
_2rdacarrier
504 _aIncluye bibliografía.
505 0 _aV. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- v. 2 : Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- v. 3 : Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies.
650 1 4 _9310488
_aClonación molecular
_xManuales de laboratorio.
700 1 _9272184
_aRussell, David William,
_d1957- .
_eautor
942 _2ddc
_cGEN
991 _aC0
_bUN@
991 _aCB
997 _aHZ
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